Overarching project required a 60-mer primer with six degenerate bases, each primer being a pool of 4096 oligonucleotides

Involved in the development and optimization of a convenient and cost-effective protocol for purification of these degenerate primers on SPE and HPLC

Primarily utilized reverse phase Fluoro-Pak and FluoroFlash technology for affinity based purification on column chromatography

Utilized Agilent 1200 UPLC, SpectraMax 190 Microplate reader, and Agilent 6200 Series TOF-MS for sample testing and analysis in 96 well format at each step of purification process

Experience troubleshooting methodologies of DNA purification, extensive testing and experimentation to improve product yields

Operated under Standard Operating Procedures and company protocols, and gained some exposure to manufacturing and cGMP environments